Rapid Detection of the mt3243A > G Mutation Using Urine Sediment in Elderly Chinese Type 2 Diabetic Patients
Joint Authors
Zhang, Yinan
Jia, Weiping
Zhang, Xiaojie
Wang, Congrong
Feng, Yanmei
Geng, Xinqian
Lu, Huijuan
Du, Xiujuan
Chu, Chen
Shen, Yixie
Chen, Ruihua
Fang, Pingyan
Chen, Yan
Zhou, Yanping
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
In this study, we aimed to identify mt3243A > G mutation carriers in a group of Chinese elderly type 2 diabetic patients by a rapid and noninvasive diagnostic system.
Methods.
DNA was extracted from blood, saliva, and urine sediment samples.
The mutation screening and quantitation of heteroplasmy were performed by high-resolution melting (HRM) curve and pyrosequencing, respectively.
Patients with mt3243A > G mutation underwent a detailed audiometric, ophthalmologic, neurological, and cardiac examination.
Results.
Two patients (2/1041) carrying the mt3243A > G mutation were detected among all type 2 diabetic patients.
In patient 1, the heteroplasmy was 0.8%, 2.8%, and 14.7% in peripheral blood leukocytes, saliva, and urine sediment, respectively.
In patient 2, the heteroplasmy was 5.3%, 8.4%, and 37.7% in peripheral blood leukocytes, saliva, and urine sediment, respectively.
Both of the two patients showed hearing impairment.
Abnormal ophthalmologic conditions and hyperintensity on T2-weighted magnetic resonance images were showed in patient 1.
Conclusion.
The occurrence of mt3243 A > G mutation was 0.2% in Chinese elderly type 2 diabetic patients.
Moreover, detection of mt3243 A > G mutation in urine sediment with high-resolution melting (HRM) curve and pyrosequencing is feasible in molecular genetic diagnosis.
American Psychological Association (APA)
Zhang, Yinan& Du, Xiujuan& Geng, Xinqian& Chu, Chen& Lu, Huijuan& Shen, Yixie…[et al.]. 2017. Rapid Detection of the mt3243A > G Mutation Using Urine Sediment in Elderly Chinese Type 2 Diabetic Patients. Journal of Diabetes Research،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1174320
Modern Language Association (MLA)
Zhang, Yinan…[et al.]. Rapid Detection of the mt3243A > G Mutation Using Urine Sediment in Elderly Chinese Type 2 Diabetic Patients. Journal of Diabetes Research No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1174320
American Medical Association (AMA)
Zhang, Yinan& Du, Xiujuan& Geng, Xinqian& Chu, Chen& Lu, Huijuan& Shen, Yixie…[et al.]. Rapid Detection of the mt3243A > G Mutation Using Urine Sediment in Elderly Chinese Type 2 Diabetic Patients. Journal of Diabetes Research. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1174320
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174320